Product Info Summary
SKU: | A14226-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-SNX30 Antibody Picoband™
SKU/Catalog Number
A14226-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SNX30 Antibody Picoband™ catalog # A14226-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SNX30 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14226-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SNX30 recombinant protein (Position: D82-K437).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A14226-1 is reactive to Snx30 in Human, Mouse, Rat
Applications
A14226-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
49.52kDa
Background of Snx30
Predicted to enable phosphatidylinositol binding activity. Predicted to be involved in protein transport.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SNX30 using anti-SNX30 antibody (A14226-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX30 antigen affinity purified polyclonal antibody (Catalog # A14226-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX30 at approximately 50 kDa. The expected band size for SNX30 is at 50 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Snx30 (Source: Uniprot.org, NCBI)
Gene Name
Snx30
Full Name
Sorting nexin-30
Weight
49.52kDa
Superfamily
sorting nexin family
Alternative Names
ATG24A; FLJ26481; FLJ35589; sorting nexin family member 30; sorting nexin-30
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Snx30, check out the Snx30 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Snx30: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SNX30 Antibody Picoband™ (A14226-1)
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No publications found for A14226-1
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Customer Reviews
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